Effect of electromagnetic induction on impulse conduction in the frog nerve-muscle preparation.

Aliquots (25 pl ) were derivatized with phenylisothiocyanatc, vacuum dried and dissolved in 100 pl of 5 mwphosphatc buffer, p H 7.4, containing 5% (v/v) acetonitrile. Ten microlitres was applied to a Pico-Tag column (1 5 cm x 3.9 cm) maintained at 38°C. T h e column eluent was monitored at 254 nm. Two eluents A [70 mM-sodium acetate at pH 6.4 containing 6 % (v/v) acetonitrile] and B [60% (v/v) acetonitrile and 40% (v/v) methanol) were used to produce the following gradient: time (min), %B, flow rate (ml/min); 01 0 , 0, I ; 10-10.5, 46, 1; 10.5-11.5, 100, I ; 1I.S-l2.0, 100, 1 ; 12.0-12.5, 100, 1.5; 12.5-20,0, 1.5; 20-20.5,0, 1 .